đī¸ Protein engineering
Protein Engineering is the process of developing useful or valuable proteins by designing and producing non-natural polypeptides, usually by altering the sequence of amino acids found in nature.
đī¸ Enzyme engineering
To achieve success in enzyme discovery and optimization, it's essential to have a multifaceted approach that encompasses diversity, screening capacity, and robust analytical capabilities. The journey begins with the ability to diversify effectively, ensuring a broad exploration of potential candidates. This diversity is crucial for uncovering novel enzymes or optimal starting points for evolutionary campaigns. Equally important is having a high-throughput screening capacity with low coefficient of variation (CV), which ensures consistent and reliable results across tests. This screening capability must be paired with advanced next-generation sequencing (NGS) techniques, allowing for the comprehensive sequencing data.
đī¸ Purchase mutant library plates
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đī¸ Peptide engineering
Peptides can be optimized using a similar approach to protein and enzyme engineering. Instead of enzyme activity, the assay needs to be tailored to the phentoype of interest (such as binding to a particular target of interest). Oftentimes, the design of such assays requires a lot of ingenuity!